Journal: Chembiochem

Article Title: Isopentenol Utilization Pathway for the Production of Linalool in Escherichia coli Using an Improved Bacterial Linalool/Nerolidol Synthase

doi: 10.1002/cbic.202100110

Figure Lengend Snippet: Linalool and nerolidol titres obtained with the IU pathway using the plasmid p(iso)prenol‐GPPS‐bLinS, 12.5 mM isoprenol and prenol as substrates, NEB‐5α and NEB‐10β cells and different bLinS variants. E. coli cells were grown in 3 mL TB media with 20 % n ‐nonane overlay for 72 h. Error bars represent the standard deviation of three biological replicates.

Article Snippet: Terpenoids production was performed in phosphate buffered TB using E. coli DH5α, DH10β and BL21 (NEB 5α, NEB 10β and BL21(DE3), New England Biolabs).

Techniques: Plasmid Preparation, Standard Deviation

Journal: Chembiochem

Article Title: Isopentenol Utilization Pathway for the Production of Linalool in Escherichia coli Using an Improved Bacterial Linalool/Nerolidol Synthase

doi: 10.1002/cbic.202100110

Figure Lengend Snippet: Comparison of optimised linalool and nerolidol product titres (A) and product ratio (B) obtained with the MVA pathway (NEB‐5α cells) and the IU pathway (NEB‐10β cells, 12.5 mM each isoprenol and prenol as substrates) using different bLinS variants.

Article Snippet: Terpenoids production was performed in phosphate buffered TB using E. coli DH5α, DH10β and BL21 (NEB 5α, NEB 10β and BL21(DE3), New England Biolabs).

Techniques:

Journal: bioRxiv

Article Title: Discovery, characterization, and application of chromosomal integration sites in the hyperthermophilic crenarchaeon Sulfolobus islandicus

doi: 10.1101/2025.03.16.643552

Figure Lengend Snippet: Discovery of integration sites in S. islandicus M.16.4. (a) Computational workflow for the selection of candidate neutral integration sites. The pipeline was executed on the genome of S. islandicus M.16.4 for the endogenous Type I-A CRISPR-Cas system to identify 40-nucleotide protospacers with a ‘CCN’ PAM and at least 10 mismatches. 66 crRNAs targeting 21 intergenic regions were found suitable for gene integration. Available genomics, transcriptomics, and epigenomics data were integrated to guide the selection of 13 crRNAs targeting eight intergenic loci for characterization. (b) Multi-omics map for the integration sites located using the CRISPR-COPIES pipeline. Dashed vertical lines indicate the location of the identified integration sites along the chromosome of the M.16.4 strain. The green color highlights the sites chosen for characterization in this study. The height of solid yellow, green, and blue vertical lines represents the conservation score, log 10 (expression) of neighboring genes, and mean ClsN enrichment over approximately 150 nt mapped from REY15A data using BLAST, respectively. Red circles represent the origin of replications.

Article Snippet: The ligation mixture was transformed in NEB-10β E. coli competent cells and plated on LB agar plates supplemented with appropriate antibiotics.

Techniques: Selection, CRISPR, Expressing

Journal: Cell Genomics

Article Title: Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe

doi: 10.1016/j.xgen.2022.100248

Figure Lengend Snippet:

Article Snippet: Plasmids were amplified in NEB-10 β competent E. coli (#C3019H, New England Biolabs) and purified with the HiSpeed Plasmid Maxi Kit (#12663, Qiagen).

Techniques: Produced, Virus, Recombinant, Transfection, SYBR Green Assay, Western Blot, Protease Inhibitor, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Staining, Control, Software, Sequencing, DNA Library Preparation